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superfolder green fluorescent protein/TEM β-lactamase-modified DNA origami tiles

Based on

1 Articles
2017 Most recent source

Composition

1

pKD1

Type Biomolecule
Formula
Role framework element
2

DNA staple strands

Type Biomolecule
Formula
Role framework element
3

single-stranded DNA-conjugated superfolder green fluorescent protein mutant

ssDNA-conjugated superfolder GFP mutant sfGFP132azF-ssDNA
Type Biomolecule
Formula
Role graft
4

single-stranded DNA-conjugated TEM β-lactamase mutant

ssDNA-conjugated TEM β-lactamase mutant BL165azF-ssDNA
Type Biomolecule
Formula
Role graft

Properties

Catalytic properties

Reaction Value Nanomaterial Variant Source
nitrocefin hydrolysis

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Characterization

Method Nanomaterial Variant Source
fluorescence spectroscopy

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Preparation

Method 1

Type: Physical formation
Source:
Starting materials
  • single-stranded DNA-conjugated superfolder green fluorescent protein mutant
  • DNA origami tiles
  • single-stranded DNA-conjugated TEM β-lactamase mutant
  1. kq2hxHgeIFVWJqy6MkRXTK8nvIvVmMSu5ymsuXShg
  2. BETxxb
Product

superfolder green fluorescent protein/TEM β-lactamase-modified DNA origami tiles

Length: ~ 80 nm

Width: ~ 30 nm

Medium/Support: none

Method 2

Type: Physical formation
Source:
Starting materials
  • single-stranded DNA-conjugated superfolder green fluorescent protein mutant
  • DNA origami tiles
  • single-stranded DNA-conjugated TEM β-lactamase mutant
  1. Npvw7Yvbq9EJGNNcGIHLJpWJHcetU2TJirgdhISxE
  2. 4uiExf
Product

superfolder green fluorescent protein/TEM β-lactamase-modified DNA origami tiles

Length: ~ 80 nm

Width: ~ 30 nm

Medium/Support: none

Method 3

Type: Physical formation
Source:
Starting materials
  • single-stranded DNA-conjugated superfolder green fluorescent protein mutant
  • DNA origami tiles
  • single-stranded DNA-conjugated TEM β-lactamase mutant
  1. WQdGi5uUFyTLI7fVj2c225uXT5y0yXVC0jAtPh86M
  2. kkBbHz
Product

superfolder green fluorescent protein/TEM β-lactamase-modified DNA origami tiles

Length: ~ 80 nm

Width: ~ 30 nm

Medium/Support: none

References

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