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Alexa Fluor 488-labeled siRNA-loaded poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(dimethylamino ethyl methacrylate-co-butyl methacrylate) polyplexes

Based on

2 Articles
2017 Most recent source

Composition

1

poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(dimethylamino ethyl methacrylate-co-butyl methacrylate)

PMPC-b-P(DMAEMA-co-BMA)
Type Polymer
Formula
Role carriers
2

Alexa Fluor 488-labeled small interfering RNA

Alexa Fluor 488-labeled siRNa
Type Biomolecule
Formula
Role loaded material

Properties

General physical and chemical properties

Property Value Nanomaterial Variant Source
colloidal stability

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Applications

Area Application Nanomaterial Variant Source
genetic engineering

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Characterization

Biological effects

Biological system Test details Nanomaterial Variant Source
human epithelial breast cancer MDA-MB-231 cells constitutively expressing luciferase

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Preparation

Method 1

Type: Physical formation
Source:
Product

Alexa Fluor 488-labeled siRNA-loaded poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(dimethylamino ethyl methacrylate-co-butyl methacrylate) polyplexes

Size: not specified

Medium: FBS solution

Support: none

Method 2

Type: Physical formation
Source:
Product

Alexa Fluor 488-labeled siRNA-loaded poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(dimethylamino ethyl methacrylate-co-butyl methacrylate) polyplexes

Size: not specified

Medium: heparan sulfate solution

Support: none

Method 3

Type: Physical formation
Source:
Product

Alexa Fluor 488-labeled siRNA-loaded poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(dimethylamino ethyl methacrylate-co-butyl methacrylate) polyplexes

Size: not specified

Medium: FBS solution

Support: none

Method 4

Type: Physical formation
Source:
Product

Alexa Fluor 488-labeled siRNA-loaded poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(dimethylamino ethyl methacrylate-co-butyl methacrylate) polyplexes

Size: not specified

Medium: heparan sulfate solution

Support: none

Method 5

Type: Physical formation
Source:
Starting materials
  • Alexa Fluor 488-labeled small interfering RNA
  • poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(dimethylamino ethyl methacrylate-co-butyl methacrylate)
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  2. mvmP
Product

Alexa Fluor 488-labeled siRNA-loaded poly(2-methacryloyloxyethyl phosphorylcholine)-b-poly(dimethylamino ethyl methacrylate-co-butyl methacrylate) polyplexes

Size: not specified

Medium/Support: none

References

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