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ENTH/DOPC/PtdIns(4,5)P2 vesicles

Based on

1 Articles
2017 Most recent source

Composition

Image only illustrates the order and placement of components as described in literature.

1

EGTA/EDTA/NaCl/MOPS buffer

Type Complex Compound
Formula
Role core
2

DOPC/PtdIns(4,5)P2

Type Complex Compound
Formula
Role layer
3

Atto 594-labeled epsin N-terminal homology domain

Atto 594-labeled ENTH domain
Type Biomolecule
Formula
Role layer

Properties

Applications

Characterization

Method Source
transmission electron microscopy

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Biological effects

Preparation

Method 1

Type: Physical formation
Source:
Starting materials
  • 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine
  • (1R,2S,3R,4R,5S,6R)-2,3,5-trihydroxy-4-[({2-[(5Z,8Z,11Z,14Z)-octadeca-5,8,11,14-tetraenoyloxy]-3-(octadecanoyloxy)propoxy}phosphinato)oxy]-6-(phosphonatooxy)cyclohexyl phosphate
  • EGTA/EDTA/NaCl/MOPS buffer
Product

ENTH/DOPC/PtdIns(4,5)P2 vesicles

Method 2

Type: Physical formation
Source:
Starting materials
  • 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine
  • (1R,2S,3R,4R,5S,6R)-2,3,5-trihydroxy-4-[({2-[(5Z,8Z,11Z,14Z)-octadeca-5,8,11,14-tetraenoyloxy]-3-(octadecanoyloxy)propoxy}phosphinato)oxy]-6-(phosphonatooxy)cyclohexyl phosphate
  • EGTA/EDTA/NaCl/MOPS buffer
Product

ENTH/DOPC/PtdIns(4,5)P2 vesicles

References

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