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DOPE/DOTAP-coated ERP/luciferase plasmid DNA polyplexes

Based on

1 Articles
2016 Most recent source

Composition

Image only illustrates the order and placement of components as described in literature.

1

ERP/luciferase plasmid DNA polyplexes

ERP/pLUCI polyplexes
Type Nano Material
Formula
Role core
2

DOPE/DOTAP mixture

Type Complex Compound
Formula
Role layer

Properties

General physical and chemical properties

Property Value Nanomaterial Variant Source
Zeta potential

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Applications

Area Application Nanomaterial Variant Source
genetic engineering

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Characterization

Method Nanomaterial Variant Source
dynamic light scattering

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Biological effects

Biological system Test details Nanomaterial Variant Source
human cervix adenocarcinoma (HeLa) cells

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Preparation

Method 1

Type: Physical formation
Source:
Starting materials
  • N,N,N-trimethyl-2,3-bis[(9Z)-9-octadecenoyloxy]-1-propanaminium
  • (2-aminoethoxy)({2,3-bis[(9Z)-octadec-9-enoyloxy]propoxy})phosphinic acid
  1. xkj4N4PBRjKQw0KOm0WXE
Product

DOPE/DOTAP-coated ERP/luciferase plasmid DNA polyplexes

Hydrodynamic diameter: ~ 75 nm

Medium/Support: none

Method 2

Type: Physical formation
Source:
Starting materials
  • (2-aminoethoxy)({2,3-bis[(9Z)-octadec-9-enoyloxy]propoxy})phosphinic acid
  • N,N,N-trimethyl-2,3-bis[(9Z)-9-octadecenoyloxy]-1-propanaminium
  1. W7yOC5kpB3cKDznKRobzK
Product

DOPE/DOTAP-coated ERP/luciferase plasmid DNA polyplexes

Hydrodynamic diameter: ~ 115 nm

Medium/Support: none

Method 3

Type: Physical formation
Source:
Starting materials
  • N,N,N-trimethyl-2,3-bis[(9Z)-9-octadecenoyloxy]-1-propanaminium
  • (2-aminoethoxy)({2,3-bis[(9Z)-octadec-9-enoyloxy]propoxy})phosphinic acid
  1. iZzz3BtYq3ScCPSJqPLor
Product

DOPE/DOTAP-coated ERP/luciferase plasmid DNA polyplexes

Hydrodynamic diameter: ~ 100 nm

Medium/Support: none

Method 4

Type: Physical formation
Source:
Starting materials
  • (2-aminoethoxy)({2,3-bis[(9Z)-octadec-9-enoyloxy]propoxy})phosphinic acid
  • N,N,N-trimethyl-2,3-bis[(9Z)-9-octadecenoyloxy]-1-propanaminium
  1. zao1HomzmsDpm9SVNfcQY
Product

DOPE/DOTAP-coated ERP/luciferase plasmid DNA polyplexes

Hydrodynamic diameter: ~ 67 nm

Medium/Support: none

Method 5

Type: Physical formation
Source:
Starting materials
  • N,N,N-trimethyl-2,3-bis[(9Z)-9-octadecenoyloxy]-1-propanaminium
  • (2-aminoethoxy)({2,3-bis[(9Z)-octadec-9-enoyloxy]propoxy})phosphinic acid
  1. drnwjo28MD2GawjWV8343
Product

DOPE/DOTAP-coated ERP/luciferase plasmid DNA polyplexes

Hydrodynamic diameter: ~ 140 nm

Medium/Support: none

References

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