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polypeptide/luciferase reporter gene plasmid pGL-3 nanoparticles colloid

Based on

13 Articles
2016 Most recent source

Composition

1

poly((1.5-pentanediol diacrylate)-(3-amino-1-propanol)-(1-(3-aminopropyl)-4-methylpiperazine)

poly(beta-amino ester) PBAE
Type Polymer
Formula
Role carriers
2

enhanced green fluorescent protein-encoding deoxyribonucleic acid

eGFP DNA
Type Biomolecule
Formula
Role loaded material

Properties

General physical and chemical properties

Property Value Nanomaterial Variant Source
Zeta potential

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Applications

Area Application Nanomaterial Variant Source
drug delivery

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Characterization

Method Nanomaterial Variant Source
dynamic light scattering

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transmission electron microscopy

Biological effects

Biological system Test details Nanomaterial Variant Source
A549 cells

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Preparation

Method 1

Type: Physical formation
Source:
Starting materials
  • luciferase reporter gene plasmid pGL-3
  • xPolyR8(FA)-KLA(TPP)
  1. 1YQm1PJoDj3yYUrEpTwdjx3lcx7XKLjckMRDDDzIjZ
  2. HJuPcc
  3. Zsb
Product

polypeptide/luciferase reporter gene plasmid pGL-3 nanoparticles colloid

Diameter: ~ 20 nm

Medium/Support: none

Method 2

Type: Physical formation
Source:
Starting materials
  1. 30ekC4TylbrPKMCPh0
Product

polypeptide/luciferase reporter gene plasmid pGL-3 nanoparticles colloid

Size: not specified

Medium: potassium chloride aqueous solution

Support: none

Method 3

Type: Physical formation
Source:
Starting materials
  • PEGylated poly-L-lysine
  • green fluorescent protein-encoding plasmid driven by cytomegalovirus promoter
  1. MDwAKyB7uTNVXTIdkbZS832rPgToRb81QMfeS4x75dJEhbD
  2. S9pDHoxu
Product

polypeptide/luciferase reporter gene plasmid pGL-3 nanoparticles colloid

Size: not specified

Medium/Support: none

Method 4

Type: Physical formation
Source:
Starting materials
Product

polypeptide/luciferase reporter gene plasmid pGL-3 nanoparticles colloid

Hydrodynamic diameter: ~ 80 nm

Medium: phosphate buffer solution

Support: none

Method 5

Type: Physical formation
Source:
Starting materials
Product

polypeptide/luciferase reporter gene plasmid pGL-3 nanoparticles colloid

Hydrodynamic diameter: ~ 150 nm

Medium: phosphate buffer solution

Support: none

References

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