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poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-(2-dimethylaminoethylamine)]/shRNA complex nanoparticles

Based on

1 Articles
2011 Most recent source

Composition

Image only illustrates the order and placement of components as described in literature.

1

polyethyleneimine

polyethyleneimine PEI PAA
Type Polymer
Formula
Role carriers
2

pGPU6/Neo-GFP22-shRNA-expressing pDNA

Type Biomolecule
Formula
Role loaded material

Properties

General physical and chemical properties

Property Value Nanomaterial Variant Source
Zeta potential

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Applications

Area Application Nanomaterial Variant Source
genetic engineering

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Characterization

Method Nanomaterial Variant Source
dynamic light scattering

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Biological effects

Biological system Test details Nanomaterial Variant Source
HEK-293 cell (Human embryonic kidney cell)

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Preparation

Method 1

Type: Physical formation
Source:
Starting materials
  • polyethyleneimine
  • pGPU6/Neo-GFP22-shRNA-expressing pDNA
  1. 1fbcENjZdh3wAZ
Product

poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-(2-dimethylaminoethylamine)]/shRNA complex nanoparticles

Size: not specified

Medium/Support: none

Method 2

Type: Physical formation
Source:
Starting materials
  • poly(hexylene glycol diacrylate-β-triethylenetetramine)
  • pGPU6/Neo-GFP22-shRNA-expressing pDNA
  1. yr1wJF1kOxASJx
Product

poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-(2-dimethylaminoethylamine)]/shRNA complex nanoparticles

Size: not specified

Medium/Support: none

Method 3

Type: Physical formation
Source:
Starting materials
  • poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-triethylenetetramine]
  • pGPU6/Neo-GFP22-shRNA-expressing pDNA
  1. HwzoyUvDDQVizo
Product

poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-(2-dimethylaminoethylamine)]/shRNA complex nanoparticles

Size: ~ 60 - ~ 110 nm

Medium/Support: none

Method 4

Type: Physical formation
Source:
Starting materials
  • pGPU6/Neo-GFP22-shRNA-expressing pDNA
  • poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-diethylenetriamine]
  1. ViCF96kNM9oMGn
Product

poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-(2-dimethylaminoethylamine)]/shRNA complex nanoparticles

Size: ~ 110 - ~ 150 nm

Medium/Support: none

Method 5

Type: Physical formation
Source:
Starting materials
  • poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-(2-dimethylaminoethylamine)]
  • pGPU6/Neo-GFP22-shRNA-expressing pDNA
  1. wkgSho879GU7zP
Product

poly[bis(2-hydroxylethyl)-disulfide-diacrylate-β-(2-dimethylaminoethylamine)]/shRNA complex nanoparticles

Size: ~ 150 - ~ 200 nm

Medium/Support: none

References

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